Present study carried out to determine the effect of four weeks vitamin C supplementation with intermittent exercise on serum anti-oxidation capacity and maximal oxygen consumption in inactive women. 40 healthy inactive women after the aerobic capacity detection were randomly set in four equal groups of recipients of supplemental vitamin C (1000 mg daily for two meals a day for four weeks) (CG), placebo (lactose) (PG), interval training with placebo (three days a week, with 70% of maximum heart rate, received a five point nine minutes, four minutes passive rest) (P+TG) and interval training with vitamin C (C+TG). Initial blood sample obtained at baseline before starting supplementation and second blood sample was taken after completion of supplementation and intermittent exercise. Normal data were investigated by using one-way analysis of variance and post Bonferroni test, if significant, T in the five percent level of significance using SPSS version 22. Results: Four weeks of vitamin C supplementation on serum anti-oxidation capacity of inactive women has a significant effect (P = 0.04). But has no significant effect on maximal oxygen consumption (P = 0.11). The four-week interval training has a significant effect on serum anti-oxidation capacity (P = 0.001) and oxygen consumption (P = 0.001) on maximum inactive women. Four weeks vitamin C supplementation with intermittent exercise on serum anti-oxidation capacity (P = 0.001) and oxygen consumption (P = 0.0001) has a significant effect on inactive women. Conclusion: The results of this study also suggest that three days in a week, with 70% of maximum heart rate, received five nine minutes session, with four minutes inactive rest significantly increased serum anti-oxidation capacity and maximal oxygen is consumed. In addition, the four-week vitamin C supplementation, with promotion of serum anti-oxidation play an important role in the prevention of adverse changes in oxidative stress, membrane damage and loss in peak muscle damage after eccentric activity